![]() Recordings revealed local transmembrane depolarizations, two types of spikes with distinct fluorescence lifetimes, and phase locking of spikes to an external mechanical stimulus. Lifetime readout is limited by photon shot noise, and the method provides strong rejection of motion artifacts and technical noise sources. Lifetime resolutions of <5 picoseconds at 1 kilohertz were achieved for single-cell voltage recordings. We demonstrated that wide-field electro-optic fluorescence lifetime imaging microscopy (EO-FLIM) allows lifetime imaging at kilohertz frame-acquisition rates, spatially resolving action potential propagation and subthreshold neural activity in live adult Drosophila. Existing approaches fail to achieve the speed and sensitivity required for voltage imaging in neuroscience applications. The development of voltage-sensitive fluorescent probes suggests fluorescence lifetime as a promising readout for electrical activity in biological systems. Undergraduate Research BIO 199 (Aut, Win, Spr, Sum).Teaching Practicum in Biology BIO 290 (Aut, Win, Spr).Research PHYSICS 490 (Aut, Win, Spr, Sum).Practical Training APPPHYS 291 (Aut, Spr, Sum).Out-of-Department Undergraduate Research BIO 199X (Sum).Out-of-Department Directed Reading BIO 198X (Sum).Graduate Research NEPR 399 (Aut, Win, Spr, Sum).Graduate Research BIOPHYS 300 (Aut, Win, Spr, Sum).Graduate Research BIO 300 (Aut, Win, Spr, Sum).Directed Study BIOE 391 (Aut, Win, Spr, Sum).Directed Studies in Applied Physics APPPHYS 290 (Aut, Win, Spr, Sum).Directed Reading in Neurosciences NEPR 299 (Aut, Win, Spr, Sum).Directed Reading in Biophysics BIOPHYS 399 (Aut, Win, Spr, Sum).Directed Reading in Biology BIO 198 (Aut, Win, Spr, Sum).Directed Investigation BIOE 392 (Aut, Win, Spr, Sum).Bioengineering Problems and Experimental Investigation BIOE 191 (Aut, Win, Spr, Sum).Introduction to Biophysics APPPHYS 205, BIO 126, BIO 226 (Win).Advanced Imaging Lab in Biophysics APPPHYS 232, BIO 132, BIO 232, BIOPHYS 232, GENE 232 (Spr).Our research emphasizes understanding the control and learning of motor behaviors, as well as the potential application of our newly developed imaging techniques to clinical use in humans. We work with both genetic model organisms, mice and fruit flies, and human subjects. We seek explanations that span different levels of organization, from cells to entire circuits. ![]() En route, we are also performing experiments on circuit properties in anesthetized animals, such as the studies that use our newly invented fluorescence endoscopes for examining hippocampal cells and dendrites in vivo. By necessity, we aim to advance imaging methods so that we can examine dynamics of neuronal populations or of dendritic compartments in behaving animals. Our approach combines behavioral, electrophysiological, and computational methodologies with high-resolution fluorescence optical imaging that is capable of resolving individual neurons and dendrites. in cognitive psychology) or more microscopic (e.g. By contrast, much current research on learning and memory concentrates on levels of organization in the nervous system that are either more macroscopic (e.g. ![]() The long-term goal of our research is to advance experimental paradigms for understanding normal cognitive and disease processes at the level of neural circuits, with emphasis on learning and memory processes.
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